Fig. 3: Irradiation induced changes in gene expression in early stationary phase.

a ‘Volcano’ plot of differentially expressed genes in cells 1 day after irradiation with 5 Gy at 0.5 Gy/min relative to control (non-irradiated) cells. Highlighted genes were significantly up-regulated (266 genes) or down-regulated (144 genes) following irradiation. b The number of genes in different gene classes with significant (false discovery rate (FDR) < 0.05) up- or down-regulation of expression and the fold-change of their expression. c Relative expression of selected lipid metabolism genes in response to irradiation as determined by qPCR and shown relative to the control (non-irradiated) expression. Numbers in square brackets represent isoforms. d The mapping of the enzymes associated with the key lipid metabolism genes that were up-regulated in response to ionizing radiation. (e) Relative expression of three transcription factor genes as determined by qPCR and shown relative to the control (non-irradiated) expression f Relative expression of four reactive oxygen species response genes as determined by qPCR and shown relative to the control (non-irradiated) expression. All data (in c, e, f) are means ± standard error and the number of biologically independent experiments was n = 3. The differences compared to control treatments are all significant at p < 0.05 as evaluated using non-parametric two-tailed Mann–Whitney U tests.