Fig. 2: Evaluation of the physiological toxicity of lactic acid and 2DG on zebrafish larvae. | Communications Biology

Fig. 2: Evaluation of the physiological toxicity of lactic acid and 2DG on zebrafish larvae.

From: Histone lactylation-ROS loop contributes to light exposure-exacerbated neutrophil recruitment in zebrafish

Fig. 2

a The chemical formula of lactic acid. b Experimental procedure for the effects of lactic acid and 2DG on zebrafish growth and development. c Heartbeat of 48 hpf zebrafish larvae (beats per minute, n = 15). d Heartbeat of 72 hpf zebrafish larvae (beats per minute, n = 15). e Survival rate curve of zebrafish larvae from 0 to 96 hpf (% survival, n = 15). f Tail swing frequency of 30 hpf zebrafish larvae (swing per minute, n = 15). g Statistical graph of body length of 96 hpf zebrafish larvae (mm, n = 15). h, i Morphological images of 96 hpf zebrafish larvae observed under a stereo microscope (scale bar: 350 μm). There were no significant differences in morphology between the lactic acid- and 2DG-treated groups compared to the control group. Data were analyzed using one-way ANOVA and log-rank test. All experiments were repeated three times. Bar graphs represent the mean ± standard error of the mean (SEM). (*p < 0.05; **p < 0.01; ***p < 0.001).

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