Fig. 5: ROS promote H3K18 lactylation and lactic acid metabolism. | Communications Biology

Fig. 5: ROS promote H3K18 lactylation and lactic acid metabolism.

From: Histone lactylation-ROS loop contributes to light exposure-exacerbated neutrophil recruitment in zebrafish

Fig. 5

a, b Fluorescence staining experiments revealed a significant increase in reactive oxygen species (ROS) levels in zebrafish larvae upon the addition of 100 μM hydrogen peroxide (n = 15). c Q-PCR results showed a significant upregulation of the oxidative stress gene duox upon treatment with 100 μM hydrogen peroxide. d, e The addition of 100 μM hydrogen peroxide significantly reduced the expression of the histone delactylation genes hdac3 and hdac12. f The expression of the histone lactylation gene ep300a was significantly upregulated. g, h Treatment with 100 μM hydrogen peroxide significantly increased the level of histone lactylation (H3K18). i, j Fluorescence staining experiments showed a significant inhibition of reactive oxygen species (ROS) levels in zebrafish larvae under LL condition upon treatment with 10 μM DPI. k Treatment with 10 μM DPI significantly reduced the expression level of the oxidative stress gene duox. l, m DPI treatment significantly inhibited the H3K18 lactylation under LL condition. Data were statistically analyzed using unpaired t tests and one-way ANOVA. All experiments were repeated three times. Bar graphs represent the mean ± standard error of the mean (SEM). (*p < 0.05; **p < 0.01; ***p < 0.001).

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