Fig. 6: ROS and Akt regulate neutrophil recruitment under LL condition.

a–c Western blotting results showed that MK-2206 significantly inhibited the expression of Akt. d The image showed that neutrophils migrated to the damaged caudal fin 3 h after injury after treatment with DPI. e The statistical graph shows that MK-2206 and DPI significantly inhibited the recruitment of neutrophils to the injured site of the caudal fin under LD condition (n = 20). f The image showed that neutrophils migrated to the damaged caudal fin 3 h after injury after treatment with MK-2206 and DPI. White rectangles represent counting areas (scale bar: 200 μm). g The statistical graph shows that MK-2206 and DPI significantly inhibited the recruitment of neutrophils to the injured site of the caudal fin under LL condition (n = 20). h The image showed that after treatment with MK-2206 and DPI, neutrophils migrated to the otic vesicle inflammation site 3 h after injury. White rectangles represent counting areas (scale: 200 μm). i The statistical graphs show that MK-2206 and DPI significantly inhibited the recruitment of neutrophils to the otic vesicle inflammation under LL condition (n = 20). The data were analyzed using unpaired t tests and one-way ANOVA. All experiments were repeated three times. Bar graphs represent the mean ± standard error of the mean (SEM). (*p < 0.05; **p < 0.01; ***p < 0.001).