Table 1 Binding characteristics and thermal stabilities of Af2 variants

From: Ubiquitin-derived artificial binding proteins targeting oncofetal fibronectin reveal scaffold plasticity by β-strand slippage

 

DSF

ELISA

SPR

Affilin a,d

Tm

KD

KD

kon

koff

 

[°C]

[nM]

[nM]

[1/M·s]

[1/s]

Af2p

53

7.7

17.1

5.7·105

9.8·10−3

Af2s

60

3.1

3.7

3.0·105

1.1·10−3

Af2

62

3.6

1.5

1.4·106

2.2·10−3

Af2s-Y141A

62

–

83.5

1.1·106

8.8·10−2

Af2s-ΔDP-KS

69

–

0.7

1.9·106

1.4·10−3

Af2-IL-2

–

1.5

2.1

1.1·106

2.3·10−3

Target b,d

     

67B89

–

3.6

–

–

–

7B8

–

2.6

–

–

–

67B

–

n.b.c

–

–

–

B89

–

2.1

–

–

–

6789

–

n.b.c

–

–

–

  1. aBinding to target 67B89.
  2. bBinding to Affilin Af2.
  3. cNo binding detected.
  4. d– indicates ‘not measured’.
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