Fig. 5: Chemical inhibition of Acs2 and V-type ATPase increases susceptibility to a variety of AFDs. | Communications Biology

Fig. 5: Chemical inhibition of Acs2 and V-type ATPase increases susceptibility to a variety of AFDs.

From: Vitamin B5 metabolism is essential for vacuolar and mitochondrial functions and drug detoxification in fungi

Fig. 5

a Schematic of the relevant portions of the PCA pathway. The vacuolar structure was created using BioRender (BioRender.com). b The growth of acs2 tet-off strain is inhibited by increasing concentrations of doxycycline. S. cerevisiae acs2 tet-off strain was inoculated in the presence or absence of doxycycline and normalized to DMSO-treated wells (no drug=100% growth) and 200 µM amorolfine (0% growth). c AR-12 potentiates caspofungin, fluconazole, and terbinafine by factors of ~100x against S. cerevisiae WT. d Concanamycin A increases yeast susceptibility to antifungal drugs. S. cerevisiae WT was inoculated in the presence or absence of concanamycin A at 30 °C for 48 h. The growth was normalized to DMSO-treated wells (no drug = 100% growth) and 200 µM amorolfine well (0% growth). Liquid growth assays were conducted in quadruplicate (n = 4) and the plotted graphs represent the average of 4 data sets ± SD.

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