Fig. 8: Uncultured Ichthyosporea groups are found in diverse environmental samples.

Maximum-likelihood phylogeny of the 324 EukBank96 amplicon sequence variants (ASVs) classified as Ichthyosporea (in black) in the context of the 116 Ichthyosporea species representatives clustered at 97% sequence identity (in green) inferred using the GTR + FO + R7 model of evolution (alignment length: 1728 nt). Read counts, normalized per environment category, for each ASV are indicated by bars colored according to the legend (See Supplementary Data 4 for ASV information and the Fig. 6 legend for a description of each environment category). Support from ultrafast bootstraps (ufboot) and non-parametric bootstraps (BP) is indicated by circles according to the legend. Branches with less than 90% ufboot support are collapsed. The outgroup and sequence IDs can be found in the labeled phylogeny in Supplementary Data 6, and alignment and tree files in Supplementary Data 5.