Fig. 1: CpG site changes during T-cell differentiation.
From: Development of an epigenetic clock resistant to changes in immune cell composition
A Experimental design for determining impact of CD8+ differentiation on epigenetic clock age prediction. B UMAP dimensionality reduction of CD8+ DNA methylation profiles. C Differences between predicted epigenetic age as a function of donor age and CD8+ T-cell subset. D Comparison of shared CpG site changes between age in CD8+ T cells and CD8+ cell subset. E Percent of sites in four epigenetic clocks that are correlated with CD8+ T-cell differentiation. Comparison of the F Hannum (p = 1.1 * 10−7), G Horvath (p = 0.001), H Horvath skin and blood (p = 2.8 * 10−6), and I PhenoAge (p = 4.8 * 10−8) epigenetic age acceleration predictions for four CD8+ T-cell subsets. *** ANOVA p-value less than or equal to 0.001. Samples derived from N = 7 individuals. Boxplots are centered at median and bound one quartile on each side.
