Fig. 2: Rearrangement of P2 and activity of P1–P3.
From: A short-lived peptide signal regulates cell-to-cell communication in Listeria monocytogenes
a UPLC-based assessment of the rearrangement rate of P2 to give P3. b Plot of the decaying concentration of P2 from a starting concentration of 1 μM (based on the determined t1/2 = 1.3 min). c Effect of selected concentrations of P1–P3 on the agr activity in luminescence-based reporter strain WT::P2-lux of L. monocytogenes grown at 37 °C in tryptic soy broth (TSB) medium. d Effect of selected concentrations of P1–P3 on the agr activity in luminescence-based reporter strain ΔagrD::P2-lux of L. monocytogenes grown at 37 °C in tryptic soy broth (TSB) medium. Shadings on the graphs represent the standard error of the mean (SEM). All reporter strain assay data is based on three individual assays (n = 3) performed in at least technical duplicate. “No peptide” control wells contain pure TSB medium corresponding to the volume added for the dilution series of the peptide. “No peptide +1% DMSO” control wells contain 1% DMSO, corresponding to the concentration of DMSO at the highest concentration of synthetic peptide (50 μM). Part of the figure was prepared using BioRender. Figure adapted from the PhD thesis of B. S. Bejder entitled “Chemical Microbiology Investigations of Peptide-based Cell-to-Cell Communication in Gram-positive Bacteria” (University of Copenhagen, 2024).
