Fig. 3: Chemical structures of peptide analogues 1–5, rearrangement evaluation of 1, and selected reporter strain assay data.
From: A short-lived peptide signal regulates cell-to-cell communication in Listeria monocytogenes
a Structural modification to give 1 and effect of selected concentrations of 1 on the agr activity in luminescence-based reporter strains of L. monocytogenes grown at 37 °C in tryptic soy broth (TSB) medium. *Based on two individual assays (n = 2) performed in technical triplicate. b HPLC analysis of the stability of 1 after incubation for 22 h in neutral buffer. c Overview of the effects of alanine substitution of compound 1. d Effects of selected concentrations of 2 and 4 on the agr activity in luminescence-based reporter strains of L. monocytogenes grown at 37 °C in tryptic soy broth (TSB) medium, representing three individual assays (n = 3) performed in at least technical duplicate. “No peptide” control wells contain pure TSB medium corresponding to the volume added for the dilution series of the peptide. Shadings on the graphs represent the standard error of the mean (SEM).
