Fig. 4: Chemical structures of peptide analogues 6–10, rearrangement evaluation of 8, and selected reporter strain assay data.
From: A short-lived peptide signal regulates cell-to-cell communication in Listeria monocytogenes
a–c Structural modifications to give 6–8 and effect of selected concentrations of 6–8 on the agr activity in the reporter strains of L. monocytogenes. d HPLC trace showing the degree of rearrangement and hydrolysis of 8 after incubation in neutral buffer for 22 h. e, f Structural modifications to give 9 and 10 and effect of selected concentrations of 9 and 10 on the agr activity in the reporter strains of L. monocytogenes. Data is based on assays performed at 37 °C in tryptic soy broth (TSB) medium, representing three individual assays (n = 3) performed in at least technical duplicate. “No peptide” control wells contain pure TSB medium corresponding to the volume added for the dilution series of the peptide. Shadings on the graphs represent the standard error of the mean (SEM).
