Fig. 5: Effect of synthetic AIP variants on agr-regulated gene expression assessed by quantitative real-time PCR (qPCR). | Communications Biology

Fig. 5: Effect of synthetic AIP variants on agr-regulated gene expression assessed by quantitative real-time PCR (qPCR).

From: A short-lived peptide signal regulates cell-to-cell communication in Listeria monocytogenes

Fig. 5

Relative quantification of agrB (a) or lmo0477 (b) expression at late exponential growth phase (6 h) in L. monocytogenes EGDeΔagrD grown with or without selected synthetic peptides [P1, P2, P3, or 10 (all added at 250 nM final concentration)] and compared with expression levels in L. monocytogenes EGDe WT. Performed in biological triplicate (n = 3). Error bars represent standard deviation. Statistical significance was evaluated by a one-way ANOVA: * (p < 0.05), ** (p < 0.005), *** (p < 0.005), **** (p < 0.0001).

Back to article page