Fig. 2: Alternative end-joining (A–EJ)-mediated translocation of the amp gene.
From: High-complexity of DNA double-strand breaks is key for alternative end-joining choice
A Circos plots of genome-wide translocation landscape of the amp gene in particle irradiation. Circles from the outside to the inside are GC skew (green), GC ratio (blue to red), gene density (blue to red), and chromosome (gray) with the micro-homology of junctions (red barplot). Individual amp translocations are represented as arcs originating from the llla (lacI::lacI::lacO::amp) site and terminating at the partner site. B Proportional distribution of the fusion patterns of the amp gene into the encoding genes in translocation targets, classified as upstream fusion, downstream fusion, and promoter fusion. C Proportional distribution of the intact fusion and missing fusions of the amp gene with encoding genes in translocation targets. D Proportional distribution of the lengths of micro-homologous sequences in translocation junctions (ns, P ≥ 0.05; *P < 0.05; **P < 0.01). E Violin plot of the proportional distribution of GC content in micro-homologous junctions, which includes median (dark gray dotted line) and the lower and upper quantiles (light gray dotted line) of the data (ns, P ≥ 0.05). F Proportion of amp translocation in recB- mutant following carbon irradiation (**P < 0.01). G Circos plots of genome-wide mutation landscape in AmpR strains subjected to carbon irradiation. Translocations/deletions (SV) are indicated by red fonts, while InDel and SNP are represented by black fonts. H Proportional distribution of three types of genome-wide mutations in bacteria irradiated by carbon ions (ns, P ≥ 0.05).
