Fig. 3: Suppressive effect of homology on amp translocation. | Communications Biology

Fig. 3: Suppressive effect of homology on amp translocation.

From: High-complexity of DNA double-strand breaks is key for alternative end-joining choice

Fig. 3

A Translocation reporter strains with psgRNA plasmid that harbors a 44 bp homologous sequence of the amp gene (top, 44 bp sequence indicated in orange) and pl4sgRNA that harbors the 44 bp sequence and an additional 465 bp flanking sequence (bottom, 465 bp sequence indicated in blue). B Carbon irradiation-induced mutation rates of AmpR in the strains MG1655TR (psgRNA) and MG1655TR (pl4sgRNA) (n = 5; **P < 0.01). C Proportional distribution of the three patterns of the amp activation in AmpR clones, in which the percentage of the amp translocation is zero.

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