Fig. 1: Growth kinetics of ompA-like mutants.

The WT strain AB5075 (WT), mutant, and complemented strains were grown with shaking at the following conditions: A at 37 °C in LB broth for 8 h (n = 10); B at 42 °C in LB broth for 3 h (n = 3); and C at 37 °C on LB agarose 1% for 15 h (n = 9). OD600 values of each strain were recorded hourly, whereas CFU/ml was measured at the endpoint. Data are shown as means ± SDs. Statistical significance of one-way- and two-way ANOVA (color-code asterisks): *p < 0.05, **p < 0.01, and ***p < 0.001.