Fig. 4: Stress tolerance of ompA-like mutants.

The effect of ompA-like mutations on tolerance to growth stresses was evaluated on agar plates supplemented with A ethanol 4% (n = 3), B pH 6.0 (n = 3), C NaCl 20 g/l (n = 3), D Triton X-100 1% (n = 5), E chlorpromazine 16 µg/ml (n = 3), F zeocin 25 µg/ml (n = 3), G meropenem 4 µg/ml (n = 4). H Tolerance to human serum was evaluated by exposing bacteria (5 × 105 CFU/ml) to 100% human serum and incubating at 37 °C for 2 h and 4 h before CFU/ml counting (n = 4). Data (log₁₀) are shown as means ± SDs. Statistical significance was evaluated by one- and two-way ANOVA.