Fig. 4: Linoleic acid diols activate calcium flux in cultured adipocytes.

X-axis is timepoint after baseline (baseline is timepoint zero). Each timepoint is approximately 50 seconds apart. Immediately after baseline, vehicle control or different concentrations of linoleic acid diol were injected. Y-axis is FLUOFORTE fluorescence quantification in arbitrary units (A.U.). To normalize, values were divided by their mean baseline value. Each data point is from an independent well; n = 3 independent wells per group. a Differentiated WT1 brown adipocytes treated with 12,13-diHOME or vehicle control. b Differentiated WT1 brown adipocytes treated with 9,10-diHOME or vehicle control. c Differentiated 3T3-L1 white adipocytes treated with 12,13-diHOME or vehicle control. d Differentiated 3T3-L1 white adipocytes treated with 9,10-diHOME or vehicle control. For each panel, normalized post-baseline fluorescence was regressed on continuous timepoint and categorical dose; each dose was compared to vehicle control with a two-sided t-test; and p-values from all panels were Bonferroni corrected. ***Bonferroni-corrected P < 0.001 vs. vehicle control. Statistical results are in Supplementary Data 1.