Fig. 5: Treatment of 3D Liver Organoids with NAD+. | Communications Biology

Fig. 5: Treatment of 3D Liver Organoids with NAD+.

From: Extracellular NAD+ response to post-hepatectomy liver failure: bridging preclinical and clinical findings

Fig. 5

a A formation of hepatocyte spheroids fixated in formaldehyde and stained with hematoxylin and eosin. b Cell viability of these spheroids, divided into 4 treatment groups (NAD+ treatment, NAD+ & TNFalpha treatment, control and TNFalpha treatment) assessed on pre-specified timepoints via luminescence, statistically analysed with two-way ANOVA. Error lines represent standard deviation. c Cell specificity and formation demonstrated with Alexa594 conjugated Albumin and d APC coupled Cytokeratin 18 immunofluorescence staining with both DAPI counterstaining, and their corresponding negative controls.

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