Fig. 3: Activating intraosseous lymphatic vessels counteracts bone resorption. | Communications Biology

Fig. 3: Activating intraosseous lymphatic vessels counteracts bone resorption.

From: Preventing periprosthetic osteolysis in aging populations through lymphatic activation and stem cell-associated secretory phenotype inhibition

Fig. 3

a Micro-CT images illustrating cranial bone resorption from both superior and inferior perspectives after continuous TAP treatment for 7 or 14 days, concomitant with subcutaneous injection of recombinant VEGF-C. b TRAP staining in mouse cranial bones following TAP implantation, combined with recombinant VEGF-C injection. Scale bars: 100 μm. c H&E staining in mouse cranial bones following TAP implantation, combined with recombinant VEGF-C injection. Scale bars: 100 μm. d IF staining for DAPI with LYVE1 (green) and PROX1 (red). Scale bars, 100 μm. e Quantification of the lytic area in calvarial bone tissues analyzed by micro-CT (n = 6). f Quantification of TRAP-stained areas in calvarial bone sections (n = 6). g Quantification of inflammatory infiltrating cells in calvarial bone sections (n = 6). h, i The corresponding quantitative data for LYVE1 (green) and PROX1 (red) expressions in cranial bones on day 7 or day 14 after combined TAP and recombinant VEGF-C treatments (n = 6). Data are shown as means ± SD. Significance was assessed through one-way ANOVA.

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