Fig. 4: Therapeutic effect of VEGF-C on pathological bone loss induced by LPS and TNF-α administration. | Communications Biology

Fig. 4: Therapeutic effect of VEGF-C on pathological bone loss induced by LPS and TNF-α administration.

From: Preventing periprosthetic osteolysis in aging populations through lymphatic activation and stem cell-associated secretory phenotype inhibition

Fig. 4

a Micro-CT images depict cranial bone resorption from both superior and inferior perspectives after continuous LPS treatment for 14 days, concomitant with subcutaneous injection of recombinant VEGF-C. b TRAP staining in mouse cranial bones following LPS treatment, combined with recombinant VEGF-C injection. Scale bars: 100 μm. c H&E staining in mouse cranial bones following LPS treatment, combined with recombinant VEGF-C injection. Scale bars: 100 μm. d IF staining for DAPI with LYVE1 (green) and PROX1 (red). Scale bars, 100 μm. e Quantification of the lytic area in calvarial bone tissues analyzed by micro-CT (n = 6). f Quantification of TRAP-stained areas in calvarial bone sections (n = 6). g Quantification of inflammatory infiltrating cells in calvarial bone sections (n = 6). h, i The corresponding quantitative data for LYVE1 (green) and PROX1 (red) expressions in cranial bones on day 14 after combined LPS and recombinant VEGF-C treatments (n = 6). j Micro-CT images depict cranial bone resorption from both superior and inferior perspectives after continuous TNF-α treatment for 14 days, concomitant with subcutaneous injection of recombinant VEGF-C. k TRAP staining in mouse cranial bones following TNF-α treatment, combined with recombinant VEGF-C injection. Scale bars: 100 μm. l H&E staining in mouse cranial bones following TNF-α treatment, combined with recombinant VEGF-C injection. Scale bars: 100 μm. m IF staining for DAPI with LYVE1 (green) and PROX1 (red). Scale bars, 100 μm. n Quantification of the lytic area in calvarial bone tissues analyzed by micro-CT (n = 6). o Quantification of TRAP-stained areas in calvarial bone sections (n = 6). p Quantification of inflammatory infiltrating cells in calvarial bone sections (n = 6). q, r The corresponding quantitative data for LYVE1 (green) and PROX1 (red) expressions in cranial bones on day 14 after combined TNF-α and recombinant VEGF-C treatments (n = 6). Data are shown as means ± SD. Significance was assessed through one-way ANOVA.

Back to article page