Fig. 2: An optimal lipid-isopropanol concentration during SALB is critical for complete SLB coverage and minimal TXTL nonspecific adsorption. | Communications Biology

Fig. 2: An optimal lipid-isopropanol concentration during SALB is critical for complete SLB coverage and minimal TXTL nonspecific adsorption.

From: Cell-free expression with a quartz crystal microbalance enables rapid, dynamic, and label-free characterization of membrane-interacting proteins

Fig. 2

a Experimental steps of SALB formation (steps I–IV) followed by the nonspecific adsorption characterization with a TXTL reaction that only synthesizes the T7 RNA polymerase (step IV, plasmid P70a-T7rnap 0.15 nM). b The adsorption kinetics of a blank TXTL reaction (plasmid P70a-T7rnap, 0.15 nM) depend on the EggPC phospholipids concentration in IPA during step II. The cartoon frames on the right explain the different levels of nonspecific adsorption to the SLB-sensor system. c Changes in frequency after 8 hours of incubation (blank TXTL reaction with only P70a-T7rnap, 0.15 nM) as a function of phospholipids concentration in IPA step II for EggPC. d–f Same as c. for DOPC, DOPE, and E. coli lipids (ECL) total respectively. The bar level and error bars are the mean and standard deviation of at least n = 5 independent experiments.

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