Table 1 Comparison of techniques for cell-free synthesized IIMP characterization

From: Cell-free expression with a quartz crystal microbalance enables rapid, dynamic, and label-free characterization of membrane-interacting proteins

 

Techniques for characterizing IIMPs with TXTL

SDS PAGE

Microscopy

AFM

SPR

QCMa

Label-free

✓

X

✓

✓

✓

Real-time TXTL

X

✓106

X

✓107

✓

Spatially resolved

X

✓108

✓109

Xb 110

X

IIMPs interactions

✓111

✓112

✓113

✓114

✓

SLB complexityc

✓31

✓31

✓31

✓31,115

✓

Sensitivity

100 ng116

SMd117

SMd118

1 ng119

1 ng

Penetration depth

N/A

N/A

10 nm120

200 nm121

250 nm122

Preparation time

2 d123

3 h124

1 d23

1 de 125

3 h

  1. aThe information for QCMD is from this study.
  2. bSPR can have spatial resolution, but this modality is not used for IIMP characterization.
  3. cThe supported lipid bilayer (SLB) complexity refers to whether any type of lipid compositions can be achieved.
  4. dSingle molecule (SM).
  5. ePotentially, the preparation time of SPR can be reduced to the same time as QCM experiments, but no studies utilizing the SALB-TXTL approach with SPR have been reported.
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