Fig. 3: The pCyCBE base editing plasmid mediating efficient C → T conversion in Synechocystis sp. PCC 6803.
From: Development of a base editor for convenient and multiplex genome editing in cyanobacteria
a HlyD Q58 and DesB Q77 in the genome of Synechocystis sp. PCC 6803 are successfully mutated to stop codon; PAM sites are highlighted in blue color, while mutation sites are shown in red color. Each base editing experiment is repeated three times, and the representative sequencing chromatogram for each mutation is shown. b Deep sequencing results of the hlyD and desB mutant strains. The top 6 most frequent PCR products are displayed. c Growth curves of the wild-type (WT) strain, wild-type strain carrying the pCyCBE plasmid (WT + P), and the hlyD mutant (Mut hlyD) strain grown under standard Fe and Fe-deficient conditions. Photographs of the cultures on the 6th day are presented at the bottom of the growth curve. The growth curve experiments are performed in triplicate, and the data are represented as mean ± SD. d Phototactic movements of the WT strain and Mut hlyD strain grown for 7 days under lateral illumination.
