Fig. 5: Efficient multiplex editing in cyanobacteria, mediated by pCyCBE plasmid.
From: Development of a base editor for convenient and multiplex genome editing in cyanobacteria
a The pCyCBE plasmid enables simultaneous base editing at two sites. A Map of pCyCBE-2xsgRNA plasmid is presented in the left, and the representative sequencing chromatograms of two mutation sites are presented in the right. b The pCyCBE plasmid enables simultaneous base editing at three sites. Map of pCyCBE-3xsgRNA plasmid is presented in the left, and the representative sequencing chromatograms of three mutation sites are presented in the right. The mutated Cs are shown in red color and labeled with red squares.
