Fig. 6: Curing of the pCyCBE plasmid in cyanobacteria after editing.
From: Development of a base editor for convenient and multiplex genome editing in cyanobacteria
a Photographs of the edited strains grown on the BG11 agar plate in the absence and presence of sucrose. b PCR results of the six colonies randomly chosen from the BG11 plates with or without sucrose. Here, P is the pCyCBE plasmid; WT is the wild-type strain; WT + P is the wild-type strain containing pCyCBE plasmid. c All six colonies chosen from the sucrose plate are sensitive to the antibiotic (kanamycin).
