Fig. 7: The pCyCBE base editing system can inactivate most genes in the cyanobacteria.
From: Development of a base editor for convenient and multiplex genome editing in cyanobacteria
a HetF Q575 in the genome of Anabaena sp. PCC 7120 is successfully mutated to stop codon. b Photographs of Anabaena sp. PCC 7120 wild-type (WT) strain, wild-type strain carrying the pCyCBE plasmid (WT + P), and the hetF mutant strain (Mut hetF). Bars = 10 μm. c The cell lengths of different Anabaena sp. PCC 7120 strains. 100 cells from each strain are measured under microscope. ***p < 0.001 is determined using Student’s t test. d Relative position of the earliest induction of stop codons could be targeted in the ORFs of cyanobacteria (cumulative percentage) by the pCyCBE system. e The portion of the genes targeted by pCyCBE to introduce at least one premature stop codon within the top 25%, 50%, and 75% of the ORFs in Synechocystis sp. PCC 6803 and Anabaena sp. PCC 7120.
