Table 1 Comparison of different genome editing systems in cyanobacteria
From: Development of a base editor for convenient and multiplex genome editing in cyanobacteria
Conventional | Cas9 | Cpf1 (Cas12a) | Base editor | |
|---|---|---|---|---|
Editing method | Homologous recombination | Cas9-induced DSB and homologous recombination | Cpf1-induced DSB and homologous recombination | Deaminase-mediated base conversion, no DSB |
Typical length of procedure | From 3 weeks to several months until segregation | From 9 days to a few weeks until segregation | From 9 days to a few weeks until segregation | From 9 days to a few weeks until segregation |
Cloning steps | Multiple | Multiple | Multiple | One step |
Editing effect | Deletion, insertion, and mutation | Deletion, insertion, and mutation | Deletion, insertion, and mutation | Base mutation |
Completeness of gene knockout | 100% | 100% | 100% | <100% |
Homology arm | Needed | Needed | Needed | Not needed |
Multiplex editing | No | Yes | Yes | Yes |
Toxic to cyanobacteria | Low | Relatively high | Low | Low |
