Fig. 4: Differential neuro-microglial interactions post-stroke.

a Number of ligand–receptor interactions between neurons and microglia in different conditions. b Differential interactions in (i) control stroke acute vs CCR5kd acute (ii) control stroke chronic vs CREBoe chronic stroke. Red arrows denote increased no. of interactions, and blue denotes reduced no. of interactions; line thickness is proportional to no. of interactions. c, d Ligand–receptor interactions (d) from microglia to neurons and signaling pathways (c) represented by ligand–receptor interactions in treated vs control conditions. e Gene ontology terms mapped to expressed ligands and their receptors expressed across conditions. f Weighted-edge graphs showing directionality of semaphorin and thrombospondin signaling. Blue arrows from microglia to neurons and red from neurons to microglia; line thickness denotes signaling strength. g Expression of ligand–receptor genes from thrombospondin and semaphoring signaling pathways across cell types and post-stroke conditions.