Fig. 1: ROWY1 is a candidate regulatory gene for root growth and development. | Communications Biology

Fig. 1: ROWY1 is a candidate regulatory gene for root growth and development.

From: Polarly localized Bro1 domain proteins regulate PIN-FORMED abundance and root gravitropic growth in Arabidopsis

Fig. 1

a Coexpression analysis of the PIN2 gene via the ATTED-II (http://atted.jp) database identified candidate genes that may be involved in PIN2 regulation of root growth and development. b Expression patterns of ROWYs revealed by pROWY1::GUS, pROWY2::GUS, and pROWY3::GUS reporter transgenic lines in Arabidopsis roots. Five-day-old pROWYx::GUS seedlings grown on plates were selected for GUS staining and imaged via light microscopy. n = 25, 17, 20. Scale bars, 50 μm. The experiments were repeated four times independently, with similar results obtained. c Expression patterns of ROWYs revealed by pROWY1::NLS-GFP, pROWY2::NLS-GFP, and pROWY3::NLS-GFP reporter transgenic lines in Arabidopsis roots. Five-day-old pROWYx::NLS-GFP seedlings grown on plates were imaged via confocal laser scanning microscopy (CLSM). Laser wavelength, 488 nm, 5.00%; 0.0% offset; detector gain, 580 V. n = 21, 22, and 19, respectively. Scale bars, 50 μm. The experiments were repeated three times independently, with similar results obtained.

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