Fig. 5: Exogenous auxin treatment rescued the wavy root phenotype of the rowy123 mutant.

a Representative images showing the morphological changes in 7-day-old Col-0 and rowy123 seedlings grown on MS media supplemented with IAA, NAA, and 2,4-D at the indicated concentrations. DMSO was used as the solvent control. Scale bars, 1 cm. The experiments were repeated three times independently, with similar results obtained. b Exogenous auxin treatment rescued the primary root length of rowy123 to a level similar to that of Col-0. c Exogenous auxin treatment reversed the wavy root phenotype of rowy123. Seven-day-old seedlings. The data are presented as the means ± SDs; n values are indicated. P values were calculated via a two-tailed t test for the indicated pairs of Col-0 and rowy123 at the given concentrations. d, e The regular auxin-responsive pattern of DR5rev::GFP was reversed by IAA in rowy123. Four-day-old DR5rev::GFP seedlings grown on plates containing IAA at the indicated concentrations were imaged with CLSM. The dotted line circles indicate the regions used for quantification. The experiments were repeated three times independently, with similar results obtained. d Representative images of DR5rev::GFP. The fluorescence intensity is shown in dark blue, green, yellow, and white, with the fluorescence intensity increasing in turn. Laser wavelength, 488 nm, 14.00%; 0.0% offset; detector gain, 650 V. Scale bars, 20 μm. e Fluorescence intensity was measured to indicate reporter gene expression. The data are presented as the means ± SDs; n values are indicated. Different letters represent significant differences; P < 0.05; one-way ANOVA with multiple comparisons. The experiments were repeated three times independently, with similar results obtained.