Fig. 6: RNA-seq analysis revealed that ROWYs are related to the iron pathway.

a Statistics of the results of the GO enrichment analysis of genes differentially expressed between Col-0 and rowy123 plants. b Heatmap showing genes differentially expressed between Col-0 and rowy123, with upregulated genes in red and downregulated genes in blue. c–e Iron deficiency led to longer and wavy primary roots in Arabidopsis seedlings. c Representative images showing the morphological changes in 7-day-old Col-0 and rowy123 seedlings grown on iron-deficient MS media. MS medium was used as the control. Scale bars, 1 cm. d The primary root length was measured. e The gravitropic index was measured to indicate the degree of bending. Seven-day-old seedlings. n values are indicated. Different letters represent significant differences; P < 0.05; one-way ANOVA with multiple comparisons. The experiments were repeated three times independently, with similar results obtained. f, g Iron deficiency disrupted the expression of PIN2. f Representative images showing PIN2::PIN2-Venus expression in epidermal cells of seedlings grown on iron-deficient MS media. Normal MS medium was used as the control. Laser wavelength, 488 nm, 5.00%; 0.0% offset; detector gain, 650 V. Scale bars, 50 μm. g The mean Venus value was measured to indicate the expression of PIN2 in epidermal cells. The data are presented as the means ± SDs; n values are indicated. P values were calculated with a two-tailed t test. The experiments were repeated four times independently, with similar results obtained.