Fig. 5: Variation in secondary metabolite production among strains is principally driven by strain diversity. | Communications Biology

Fig. 5: Variation in secondary metabolite production among strains is principally driven by strain diversity.

From: Strain heterogeneity in a non-pathogenic Aspergillus fungus highlights factors associated with virulence

Fig. 5

A Chromatograms of the 16 strains of A. fischeri displayed three distinct profiles at both 30 °C and 37 °C temperatures. Shown are representative chromatograms of each profile. The chromatograms report retention times (x-axis) and signal intensities normalized to the highest value (y-axis) of produced metabolites; different peaks correspond to different metabolites. B Principal component analysis based on retention times and individual peak areas for different metabolites from each of the 16 strains following growth at either 30 °C or 37 °C. Approximately 50% of the variation in the chemistry profiles comes from variation between strains (PC1), and 29% of the variation is due to temperature (PC2). C Temperature-dependent differential expression of transcripts for constituent genes in each of four, virulence-associated biosynthetic gene clusters in 15 A. fischeri strains (DTO7 was excluded because of poor growth at 37 °C). Secondary metabolite (SM) presence/absence at 30 °C and 37 °C is indicated to the right of each heat map. White cells on the heatmap indicate lack of transcriptional activity. HAS hexadehydroastechrome, tryp trypacidin, bisGT bisdethiobis(methylthio)gliotoxin, GT gliotoxin, verr verruculogen, fumA fumitremorgin A, and fumB fumitremorgin B.

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