Fig. 5: The ability of starTracer to find marker genes at different expression levels.

A UMAP plot of the principal neurons in the human prefrontal cortex data, which includes 15 clusters. B The relationship between specificity level and the expression level of the top 5 marker genes. The expression level measured by \({E}_{1}\) is shown in a box plot. The specificity level of the marker genes is shown in the line chart. C–E The bubble plot of the marker genes found by starTracer using the annotation of “Sub_clust” under the \({E}_{2}\) value set as 0.0, 0.4, and 0.8. The size of the dot represents the proportion of the cells with the expression of each gene. The color means the expression level of each gene in each cell cluster.