Fig. 7: Schematic of starTracer. | Communications Biology

Fig. 7: Schematic of starTracer.

From: starTracer is an accelerated approach for precise marker gene identification in single-cell RNA-Seq analysis

Fig. 7

A The structure of the expression matrix and annotation matrix of single-cell sequencing. B starTracer provides 2 options: de-novo “searchMarker” and in-conjunction “filterMarker”. “searchMarker” requires a cell annotation matrix and an expression matrix/averaged expression matrix from a single cell experiment or a Seurat object. “searchMarker” performs max-normalize the average expression matrix, calculates the molecular index for each gene passing a threshold set by the user and outputs a matrix with marker genes. C “filterMarker” takes an output matrix form “FindAllMarkers” function, assigns genes into clusters and re-arranges them by measuring the \({T}_{i}\) for each gene. Time elapsed of “searchMarker” is much shorter than that of “FindAllMarkers” and “filterMarker”.

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