Fig. 1: SMANTIS is highly expressed in monocytes.

a SMANTIS expression in different cell types of peripheral blood mononuclear cells isolated by flow cytometry (granulocytes, n = 10; monocytes, n = 7; lymphocytes, n = 8) compared to cells cultured in cell culture (THP-1, n = 6; HUVEC, n = 7; HEK293, n = 5) as determined by RT-qPCR. Data was normalized to HUVEC (dashed line). Ordinary one-way ANOVA with Tukey post hoc test. b SMANTIS expression during differentiation of THP-1 cells treated with PMA (20 nM) for 24 h, 48 h, and 120 h followed by RT-qPCR. As control (CTL), DMSO was used. n = 5, Paired t-test. c Heat map of non-coding RNAs expressed by induced pluripotent stem cells (iPSC) differentiated to monocytes (Mono) and macrophages (M\(\varphi\)) at day 0 (d0), day 15 (d15), and day 21 (d21) as determined by RNA-Seq. The top30 altered ncRNAs are shown (up). Marker genes for iPSC (SOX2, NANOG, POU5F1), monocytes (CCR2, CX3CR1), and macrophages (CD40, CD14, CD68, CD163) show clustering of cells. Z-Score is shown on the right. n = 2. d SMANTIS expression in transcripts per million (tpm) as determined by RNA-Seq from iPSC (d0) induced differentiation to monocytes (d15), and macrophages (d21). e Acute myeloid leukemia (AML) subtypes and the respective SMANTIS expression calculated from a patient (>100) cohort (ID EGA: EGAD00001008484) shown as Box and Whiskers plot. Brown-Forsythe and Welch ANOVA with Games-Howell post hoc test. FAB, French-American-British classification; M0, minimally differentiated AML, n = 6; M1, AML without maturation, n = 24; M2, AML with maturation, n = 51; M4, myelomonocytic leukemia, n = 34; M4Eo, myelomonocytic leukemia with eosinophilia, n = 7; M5, monoblastic/monocytic leukemia, n = 12; M6, erythroleukemia, n = 1. Error bars are mean +/− SD. *p < 0.05.