Fig. 4: SMANTIS and RUNX1 limit adhesion of monocytes to endothelial cells.
From: RUNX1 interacts with lncRNA SMANTIS to regulate monocytic cell functions
a Gene ontology (GO) enrichment analysis determined with PANTHER showing pathways associated with common differentially regulated genes from SMANTIS KO and RUNX1 KO compared to control NTC. The top 10 GO terms after fold enrichment are shown. FDR, false discovery rate. b Heat map of the differentially regulated genes using Z-score associated with regulation of cell adhesion determined by RNA-Seq. Comparison of NTC, SMANTIS (SMTS) KO, and RUNX1 KO. c IGV genome browser view of the loci of ITGAL and ITGAM after CUT&RUN for RUNX1, ATAC-seq and RNA-Seq for NTC, SMANTIS KO and RUNX1 KO. Numbers in brackets indicate the number of reads. d Flow cytometry based cell adhesion assay of NTC (n = 26), SMANTIS KO (n = 21), and RUNX1 KO (n = 12) cells attached to HUVEC. Ordinary one-way ANOVA with Holm-Sidak post hoc test. e, f, g Correlation of RUNX1 (e), ITGAL (f), and ITGAM (g) expression in AML-patients expressing the lowest (n = 20) or highest (n = 20) SMANTIS levels (tpm) from EGA dataset EGAD00001008484. Mann-Whitney test. h, i RT-qPCR of ITGAM (h) or ITGAL (i) after overexpression of the SMANTIS-Alu element or an empty pcDNA3.1+ vector (CTL) in NTC or SMANTIS KO THP-1 cells. NTC overexpressing CTL were set to 1. One-Way ANOVA with Tukey’s post-hoc test. n = 4. Error bars are mean +/− SD. *p < 0.05. KO, knockout.
