Fig. 5: SMANTIS is required for the proper interaction of RUNX1 to the core-binding factor CBFB and the transcriptional co-activator protein EP300.
From: RUNX1 interacts with lncRNA SMANTIS to regulate monocytic cell functions
a Correlation of RUNX1 interaction partners CBFB, SIN3A, and EP300 expression in AML-patients expressing the lowest (n = 20) or highest (n = 20) SMANTIS levels (tpm) from EGA dataset EGAD00001008484. Mann-Whitney test. b, c, d Proximity ligation assay (PLA) of RUNX1:CBFB (b), RUNX1:SIN3A (c) and RUNX1:EP300 (d) in NTC and SMANTIS KO THP-1 cells. Representative images are shown. Yellow dots indicate interactions. Nuclei are stained with DAPI. Scale bar indicates 10 µm. e, f PLA quantification of RUNX1:EP300 (NTC, n = 24; SMTS KO, n = 25), RUNX1:SIN3A (n = 14) and RUNX1:CBFB (n = 15) interactions in nucleus (e) and cytoplasm (f) of NTC and SMANTIS KO cells. n represents quantified images from in total three experiments. Mann-Whitney test. g Z-stack imaging of the RUNX1:EP300 interaction in NTC and SMANTIS KO cells determined with PLA. Representative Z-stacks are shown. Yellow dots indicate interactions. Nuclei are stained with DAPI. Scale bar indicates 10 µm. Error bars are mean +/− SD. *p < 0.05. KO, knockout.
