Fig. 3: E2 bioPROTACs induce proteasomal degradation independently of CRL E3 ligases.
From: Targeted protein degradation using chimeric human E2 ubiquitin-conjugating enzymes
Western blots of U2OS cells transfected with mRNA encoding bioPROTACs, inactive variants or domains, and incubated in the absence (−) or presence (+) of a 5 µM MG132 for 8 h or b 1 µM MLN4294 for 24 h. Representative blots are shown from two biological replicates. Bar graphs display the mean SHP2 band densitometry relative to mock-transfected control cells (individual data points are shown for each replicate). Related to Supplementary Fig. 1.
