Fig. 4: Global proteomics analysis of E2 and VHL-based bioPROTAC expression.
From: Targeted protein degradation using chimeric human E2 ubiquitin-conjugating enzymes
Volcano plots showing data for 7657 proteins from U2OS cells transfected with either a–g bioPROTAC mRNA (wild type or mutant, as indicated) or h an siRNA pair targeting shp2 transcripts. The degradation domain (e.g. E2D1) and SHP2 are highlighted on each plot. Dashed lines indicate significance thresholds (log2FC beyond ±0.58; adjusted p value < 0.05). Data obtained from four biological replicates. i Bar graph representing the numbers and distribution of proteomic hits for the active bioPROTACs. j Circos plot for the wild type bioPROTACs. Purple lines connect common hits present in ≥2 datasets. k Heatmap displaying functional enrichment analyses for the active bioPROTACs. Datasets were individually analysed within Metascape and curated to highlight the significant changes to biological pathways for each bioPROTAC. The individual cells are coloured according to the functional enrichment score (−10logP), with darker shading indicating greater statistical significance. Related to Supplementary Figs. 5–9 and Supplementary Data 1–3.
