Fig. 4: Effect of magnesium ion or calcium ion on BCOR binding to KDM2B. | Communications Biology

Fig. 4: Effect of magnesium ion or calcium ion on BCOR binding to KDM2B.

From: Calcium modulates the tethering of BCOR-PRC1.1 enzymatic core to KDM2B via liquid-liquid phase separation

Fig. 4

A Analyzing the effect of magnesium ion on over-expressed KDM2B-Flag binding to endogenous BCOR, PCGF1 and SKP1 using Co-IP assay. The western-blotting data is representative of three independent experiments. B Relative level of BCOR or PCGF1 protein co-purified with KDM2B-Flag as shown in (A). Data are represented as mean ± SD for triplicate experiments. C Analyzing the effect of calcium on over-expressed KDM2B-Flag binding to endogenous BCOR, PCGF1 and SKP1 using Co-IP assay. The western-blotting data is representative of three independent experiments. D Relative level of BCOR or PCGF1 protein co-purified with KDM2B-Flag as shown in (C). Data are represented as mean ± SD for triplicate experiments. Expressing plasmid for KDM2B-Flag was transfected into HEK293T cells. Co-IP was performed with anti-Flag magnetic beads, after 48 h transfection. MgCl2 or CaCl2 at indicated concentration is supplemented to IP buffer.

Back to article page