Fig. 4: Effect of BES treatment on CH4 emissions from E. pulchripes, animal weight, ciliates and ciliate-associated methanogens. | Communications Biology

Fig. 4: Effect of BES treatment on CH4 emissions from E. pulchripes, animal weight, ciliates and ciliate-associated methanogens.

From: Disruption of millipede-gut microbiota in E. pulchripes and G. connexa highlights the limited role of litter fermentation and the importance of litter-associated microbes for nutrition

Fig. 4

a Emission rates of CH4 over time followed by recommence of methane production after the switch to untreated litters (indicated by the arrow). b Change in the weight of E. pulchripes over time. c Enumeration of symbiotic ciliates found in the faeces following BES treatment. d mcrA gene copy numbers in the faecal samples following BES treatment. e Fluorescence microscopy images of ciliates and the two most-abundant endosymbiotic methanogens in faecal samples of E. pulchripes using DAPI and CARD-FISH probes. ARC915: general archaea, RC281r_mod: Methanomassciillicoccales, and MB311: Methanobacteriales in the 10mM-Na-BES-treated group. f Enumeration of the methanogens associated with ciliates using FISH signals. The scale bar represents 20 µm.

Back to article page