Fig. 1: Genomic locus of the ΦMycoMar insertion.
a Shows the chromosomal position of MSMEG_4702 in M. smegmatis mc2155, with Tn7 transposons inserted between the 375th and 376th positions of the MS4702 gene via phage transduction. b Confirms the presence of the MSMEG_4702 gene in WT_Ms and ΔMSMEG_4702 cultures grown to OD600 = 0.8 through PCR amplification. c Presents the Western blot identification of Rv0102 associated with the M. smegmatis cell wall, determined by cell fractionation experiments. GroEL2 was used as a cytoplasmic marker. d Depicts the genomic context of Rv0102 in mycobacteria, highlighting conserved regions across mycobacterial genomes. Arrows indicate gene transcription. e Displays the sequence analysis of the Cu transporter Rv0102, detailing conserved functional domains and structural domains including 16 transmembrane domains. f Shows a model predicting copper binding sites based on the amino acid sequence of the Rv0102 protein.
