Fig. 6: Deletion of MMAR_0267 enhances the virulence of early M. marinum infection in zebrafish. | Communications Biology

Fig. 6: Deletion of MMAR_0267 enhances the virulence of early M. marinum infection in zebrafish.

From: Mycobacterium marinum MMAR_0267-regulated copper utilization facilitates bacterial escape from phagolysosome

Fig. 6

a Survival of Zebrafish following M. marinum infection, representative of two independent survival studies (n = 30 fish/group). b Adult wild-type zebrafish were infected with WT, ΔMMAR_0267 and ΔMMAR_0267::MMAR_0267, respectively. Each strain was adjusted to OD600 = 0.5, then 10 microliters were injected into the abdomen of zebrafish, and infection was photographed 3 days later. c Pathogenic behaviour of ΔMMAR_0267 in infection models. Bacteria load quantification of liver/spleen and skin from 6 randomly chosen surviving zebra fish from each strain at day 3 after infection using CFU estimation method (n = 3). d H&E staining of representative infected liver/spleen and skin sections at 3 days post M.marinum -infection. Arrows indicate area of neutrophil infiltration with necrosis (scale bar = 200 μmol/L).

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