Fig. 7: CFP-10 promotes STING-TBK1-IRF3 activation and inhibits cell apoptosis.

a GSEA analysis was performed on the transcriptome of zebrafish infected with WT, ΔMMAR_0267, and ΔMMAR_0267::MMAR_0267 strains. b Heat maps show related genes in glycolytic/gluconeogenesis pathways, RIG-I-like receptor signaling pathways, mTOR signaling pathways, and IgA immune signaling pathways, along with metabolomic heat maps of their signature products. Both transcriptome and metabolomic analysis were conducted on zebrafish infected with WT and ΔMMAR_0267 strains, each with three replicates. c The ΔMMAR_0267 mutant strain reduced the transcription of TBK1 and IRF3 in THP-1 cells infected with different strains. Gene expression levels were quantified by detecting STING, TBK1, and IRF3 transcripts, normalized to the internal reference gene SigA. Data represent average mRNA abundances from cells cultured in triplicates (n = 3). d TBK1 and phosphorylated TBK1 expression in THP-1 cells at 4- or 24 h post-infection were analyzed by Western blotting. Protein quantification was done using Image J software. e ELISA was used to quantitatively detect IFN-α and IFN-β levels in the liver of zebrafish infected with different strains (n = 6). f Transcription of CFP-10 in different strains at varying CuSO₄ concentrations was measured using RT-qPCR (n = 3).