Fig. 7: SPRR1B regulates the migration ability of keratinocytes.
From: SPRR1B+ keratinocytes prime oral mucosa for rapid wound healing via STAT3 activation
a Western blot of SPRR1B and internal control (GAPDH) between HOK and HaCat cells. b Western blot of SPRR1B in HOK cells expressing control shRNA or either of three independent shRNAs targeting SPRR1B. c Migrating HOK cells transduced with shRNAs of SPRR1B and shNC (control) by lentiviral delivery. Images were taken every 3 h from 0 to 12 h after scratching. d Line plot representing relative change in wound area size of HOK cells as a function of time (n = 3). e Western blot to show overexpression of SPRR1B after transfection with lentiviruses in HaCat cells. f Migrating HaCat cells transduced with SPRR1B and GFP (control) by lentiviral delivery. Images were taken every 3 h from 0 to 21 h after scratching. g Line plot representing relative change in wound area size of HaCat cells as a function of time (n = 3). Error bars are shown as mean ± SEM from n = 3. Two-way analysis of variance (ANOVA) were performed (d, g). *p < 0.05, **p < 0.01, ***p < 0.001. ns not significant.
