Fig. 3: miR-34b-5p expression affected the osteogenic differentiation of BMSCs. | Communications Biology

Fig. 3: miR-34b-5p expression affected the osteogenic differentiation of BMSCs.

From: Low frequency sinusoidal electromagnetic fields promote the osteogenic differentiation of rat bone marrow mesenchymal stem cells by modulating miR-34b-5p/STAC2

Fig. 3

a The proliferation of BMSCs after transfected with miR-34b-5p mimic or inhibitor was assessed by EdU assay. b Quantitative analysis of EdU assay. c ALP and ARS staining assays of BMSCs transfected with miR-34b-5p mimic or inhibitor. d Semi-quantitative analyses of ALP and ARS staining intensities. The quantitative analyses of staining intensities were normalized based on changes in cell number resulting from the modulation of miR-34b-5p activity. e The results of the RT-qPCR showing the expression levels of osteogenic genes following a 7-day transfection of miR-34b-5p mimic or inhibitor (OPN, ALP, RUNX2, OCN). f The results of the western blots showing the protein levels of osteogenic makers (β-catenin, Active-β-catenin, OPN, RUNX2, and ALP) after 7-day transfection of miR-34b-5p mimic or inhibitor. g Quantitative analysis of osteogenic protein expression. Data represent mean ± SD; N = 3/group; *p < 0.05; **p < 0.01; ns, not significant by one-way ANOVA.

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