Fig. 6: miR-34b-5p modulated STAC2 expression. | Communications Biology

Fig. 6: miR-34b-5p modulated STAC2 expression.

From: Low frequency sinusoidal electromagnetic fields promote the osteogenic differentiation of rat bone marrow mesenchymal stem cells by modulating miR-34b-5p/STAC2

Fig. 6

a Volcano plot depicting the differentially expressed mRNAs in BMSCs after treatment with 0.4 mT SEMFs. b Volcano plot depicting the differentially expressed mRNAs in BMSCs after treatment with 1 mT SEMFs. c Venn diagram depicting the number of commonly upregulated and downregulated mRNAs in the 0.4 mT and 1 mT groups. d Gene Ontology (GO) enrichment analysis of the commonly differentially expressed RNAs in the 0.4 mT and 1 mT groups. e Venn diagram showing the intersection between calcium ion transport-related genes from the GO database and the predicted target genes of miR-34b-5p from the TargetScan database. f potential binding sites between miR-34b-5p and STAC2 were predicted using the RNAInter web tool. g The interaction between miR-34b-5p and STAC2 was identified by dual-luciferase reporter assay. h The STAC2 protein levels and quantification were assessed in BMSCs following a 7-day exposure to low-frequency SEMFs (0.4–1 mT). i STAC2 protein level and quantification were measured in BMSCs following a 7-day transfection with miR-34b-5p mimic and miR-34b-5p inhibitor. Data represent mean ± SD; N = 3/group; *p < 0.05; **p < 0.01; ***p < 0.001; ns, not significant by one-way ANOVA.

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