Fig. 5: Validation of the interface residues of the ETBR–Gi complex in the NanoBiT Gi-protein dissociation assay.
From: Structure of endothelin ETB receptor–Gi complex in a conformation stabilized by unique NPxxL motif
Symbols and error bars represent mean and standard error of the mean (SEM), respectively, from three independent experiments, each performed in duplicate or triplicate. a–c The replaced interface residues of ETBR were examined. Data for these figures and the expression levels of WT and mutant receptors are shown in Supplementary Table 1c–e. d The replaced interface residues of Gαi were examined. Mutant Gi show luminescence counts comparable with those of WT. Data for this figure are shown in Supplementary Table 1f.
