Fig. 5: Secretome comparison between wild-type (WT) Synechocystis, a Δhfq mutant and a bloom-like culture.

a Schematic overview of Synechocystis secretome comparison resulting in low (∆hfq), medium (WT) and high (bloom-like culture) levels of protein secretion. b Venn diagram representing the overlap of protein identifications. c Comparison of Clusters of Orthologous Genes (COG) category distribution between all secreted proteins in the stable secretome and those unique to bloom conditions. d Volcano plot illustrating differential protein expression between proteins identified in the secretomes of Synechocystis in the WT and Δhfq conditions. Each dot on the plot represents an individual protein (identification derived from n = 4 biological replicates). A Student’s two-sample unpaired t-test with permutation-based multiple test correction was used for differential proteins analysis. The vertical and horizontal dashed lines represent the fold-change (plotted at −1 and 1) and significance (plotted at 0.05) thresholds, respectively. Color coding indicates significant changes (red for upregulated, blue for downregulated, and gray for non-significant changes). e Comparison of COG category distribution between all secreted proteins in the stable secretome and those in the variable secretome. COG relative percentage represents the number of times a COG ID appears in relation to the total numbers of COG IDs.