Fig. 6: Amino acids 93 and 113 affect the HA/NA balance. | Communications Biology

Fig. 6: Amino acids 93 and 113 affect the HA/NA balance.

From: Modulation of human-to-swine influenza a virus adaptation by the neuraminidase low-affinity calcium-binding pocket

Fig. 6

Viruses were normalized to 32 HAU and incubated at 4 °C for 1 h before incubation at 37 °C for the indicated time. a Elution kinetics of hVIC/11 mutants at 2 mM Ca2+. b Elution kinetics sOH/04 mutants at 2 mM Ca2+. c Elution kinetics of hVIC/11 and sOH/04 mutants at 2 mM Ca2+ and 1 μM oseltamivir. d Elution kinetics of hVIC/11 mutants at 0 mM Ca2+. e Elution kinetics sOH/04 mutants at 0 mM Ca2+. f Elution kinetics of hVIC/11 and sOH/04 mutants at 0 mM Ca2+ and 1 μM oseltamivir. g Elution time of hVIC/11 and sOH/04 mutants at 2 mM Ca2+. h Elution time of hVIC/11 and sOH/04 mutants at 0 mM Ca2+. Values are presented as the mean ± SD of two independent experiments. p values were determined by one-way ANOVA with Tukey’s multiple comparison test.

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